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p mtor ser 2448 cell signaling  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc p mtor ser 2448 cell signaling
    P Mtor Ser 2448 Cell Signaling, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 953 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+mtor+ser+2448+cell+signaling/Glu-Glu+Tag+Antibody/pmc12478157__pnas%2E2414941122%2Esapp-51-49-52
    Average 96 stars, based on 953 article reviews
    p mtor ser 2448 cell signaling - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Western Blot:

    Article Title: Rapamycin and Interleukin-1β Impair Brain-derived Neurotrophic Factor-dependent Neuron Survival by Modulating Autophagy
    Article Snippet: .. Method and dilution Akt Cell Signaling, 9272 WB, 1:1000 Atg5 Sigma, A0731 WB, 1:500 Atg7 Abcam, ab53255 WB, 1:500 Cleaved caspase-3 Millipore, 9661 WB, 1:1000 HA Cell Signaling, 2367 IFC, 1:200 LC3 Cell Signaling, 3868 WB, 1:1000; IFC, 1:200 MAP2 Abcam, ab5392 IFC, 1:2000 Pan-Neuronal Marker Millipore, MAB2300 IFC, 1:200 mTOR Cell Signaling, 2983 WB, 1:1000 p-Akt Ser-473 Cell Signaling, 4060 WB, 1:1000 p-mTOR Ser-2448 Cell Signaling, 5536 WB, 1:1000 p-p44/42 MAPK Cell Signaling, 4370 WB, 1:1000 p-S6 Ser-240/244 Cell Signaling, 5364 WB, 1:1000; IFC, 1:1000 p44/42 MAPK Cell Signaling, 9102 WB, 1:1000 p62 Cell Signaling, 5114 WB, 1:1000 S6 Cell Signaling, 2317 WB, 1:1000 -Actin Sigma, A2066 WB, 1:3000 20616 JOURNAL OF BIOLOGICAL CHEMISTRY VOLUME 289 • NUMBER 30 • JULY 25, 2014 at M em orial U niversity of N ew foundland on A ugust 1, 2014 http://w w w .jbc.org/ D ow nloaded from Transfections—The enhanced YFP plasmid was obtained from Clontech. .. Plasmids containing p70S6K (32) in competent DH5a E. coli were as follows: constitutively active p70S6K (Addgene plasmid 8993 pRK7-HA-S6K1-E389- CT), dominant negative p70S6K (Addgene plasmid 8986 pRK7-HAS6K1-F5A), wild-type p70S6K (Addgene plasmid 8984 pRK7HA-S6K1-WT), and the empty plasmid (Addgene 10883 pRK7) was used as a negative control.

    Article Title: An ErbB2/c-Src axis links bioenergetics with PRC2 translation to drive epigenetic reprogramming and mammary tumorigenesis
    Article Snippet: .. The following primary antibodies were used for immunoblotting at the indicated dilutions: H3K27me3 (C36B11) - Cell Signaling, 9733, 1/1000; Ezh2 (D2C9) XP - Cell Signaling, 5246, 1/1000; ErbB2/c-Neu (AB3) - Calbiochem, OP15, 1/500; P-Rps6 Ser 240/244 - Cell Signaling, 5364, 1/1000; P-AMPK Thr172 - Cell Signaling, 2535, 1/500; P-ACC Ser79 - Cell Signaling, 3661, 1/500; P-Src Family Kinase Tyr416 - Cell Signaling, 2101, 1/500; α-Tubulin - Cell Signaling, 2144, 1/2000; β-Actin: Sigma-Aldrich, A5316, 1/2500; c-Src (clone GD11) - Millipore, 05-184, 1/1000; 4E-BP1 - Cell Signaling, 9644, 1/1000; P-4E-BP1 Ser65 - Cell Signaling, 9451, 1/500; Rps6 - Cell Signaling, 2317, 1/1000; GAPDH - Novus, NB300-322, 1/2000; Suz12 - Cell Signaling, 3737, 1/1000; EED - Millipore, 05-1320, 1/500; RbAp46 - Cell Signaling, 6882; RbAp48 - Abcam, ab79416; AMPKα - Cell Signaling, 5832; ACC - Cell Signaling, 3676, 1/1000; Lkb1 - Cell Signaling, 3050, 1/1000; α-Tubulin - Cell Signaling, 2125, 1/2000; Raptor - Cell Signaling, 2280, 1/500; P-Raptor Ser792 - Cell Signaling, 2083, 1/250; Histone H3 - Cell Signaling, 14269, 1/1000; TSC2 - Cell Signaling, 4308, 1/1000; P-TSC2 Thr1462 - Cell Signaling, 3617, 1/500; P-TSC2 Ser1387 - Cell Signaling, 5584, 1/500; P-ErbB2 Tyr1221/1222 - Cell Signaling, 2243, 1/500; P-ErbB2 Tyr877 - Cell Signaling, 2241, 1/500; P-ErbB2 Tyr1248 - Cell Signaling, 2247, 1/500; EGFR - Cell Signaling, 2232, 1/1000; P-EGFR Tyr1068 - Cell Signaling, 3777, 1/500; ErbB3: Cell Signaling, 12708, 1/500; P-ErbB3 Tyr1289 - Cell Signaling, 4791, 1/500; Akt - Cell Signaling, 2920, 1/1000; P-Akt Ser473 - Cell Signaling, 4060, 1/1000; ERK1/2 - Cell Signaling, 9102, 1/1000; P-ERK1/2 Thr202/Tyr204 - Cell Signaling, 9101, 1/1000; Puromycin - Millipore, MABE343, 1/1000; eIF2α - Cell Signaling, 2103S, 1/1000; P-eIF2α Ser51 - Cell Signaling, 3398S, 1/1000; mTOR - Cell Signaling, 2983, 1/1000; P-mTOR Ser 2448 - Cell Signaling, 5536, 1/1000; PRAS40 - Millipore, 05-1070, 1/1000; P-PRAS40 Thr246 - Cell Signaling, 2997, 1/1000; mLST8 - Cell Signaling, 3274, 1/1000. .. Secondary antibodies used in immunoblotting at a dilution of 1/10 000 were IRDye 800CW Donkey anti-rabbit (LI-COR Biosciences, 925-32213) and IRDye 680CW Donkey anti-mouse (LI-COR Biosciences, 926-68072).

    Marker:

    Article Title: Rapamycin and Interleukin-1β Impair Brain-derived Neurotrophic Factor-dependent Neuron Survival by Modulating Autophagy
    Article Snippet: .. Method and dilution Akt Cell Signaling, 9272 WB, 1:1000 Atg5 Sigma, A0731 WB, 1:500 Atg7 Abcam, ab53255 WB, 1:500 Cleaved caspase-3 Millipore, 9661 WB, 1:1000 HA Cell Signaling, 2367 IFC, 1:200 LC3 Cell Signaling, 3868 WB, 1:1000; IFC, 1:200 MAP2 Abcam, ab5392 IFC, 1:2000 Pan-Neuronal Marker Millipore, MAB2300 IFC, 1:200 mTOR Cell Signaling, 2983 WB, 1:1000 p-Akt Ser-473 Cell Signaling, 4060 WB, 1:1000 p-mTOR Ser-2448 Cell Signaling, 5536 WB, 1:1000 p-p44/42 MAPK Cell Signaling, 4370 WB, 1:1000 p-S6 Ser-240/244 Cell Signaling, 5364 WB, 1:1000; IFC, 1:1000 p44/42 MAPK Cell Signaling, 9102 WB, 1:1000 p62 Cell Signaling, 5114 WB, 1:1000 S6 Cell Signaling, 2317 WB, 1:1000 -Actin Sigma, A2066 WB, 1:3000 20616 JOURNAL OF BIOLOGICAL CHEMISTRY VOLUME 289 • NUMBER 30 • JULY 25, 2014 at M em orial U niversity of N ew foundland on A ugust 1, 2014 http://w w w .jbc.org/ D ow nloaded from Transfections—The enhanced YFP plasmid was obtained from Clontech. .. Plasmids containing p70S6K (32) in competent DH5a E. coli were as follows: constitutively active p70S6K (Addgene plasmid 8993 pRK7-HA-S6K1-E389- CT), dominant negative p70S6K (Addgene plasmid 8986 pRK7-HAS6K1-F5A), wild-type p70S6K (Addgene plasmid 8984 pRK7HA-S6K1-WT), and the empty plasmid (Addgene 10883 pRK7) was used as a negative control.

    Transfection:

    Article Title: Rapamycin and Interleukin-1β Impair Brain-derived Neurotrophic Factor-dependent Neuron Survival by Modulating Autophagy
    Article Snippet: .. Method and dilution Akt Cell Signaling, 9272 WB, 1:1000 Atg5 Sigma, A0731 WB, 1:500 Atg7 Abcam, ab53255 WB, 1:500 Cleaved caspase-3 Millipore, 9661 WB, 1:1000 HA Cell Signaling, 2367 IFC, 1:200 LC3 Cell Signaling, 3868 WB, 1:1000; IFC, 1:200 MAP2 Abcam, ab5392 IFC, 1:2000 Pan-Neuronal Marker Millipore, MAB2300 IFC, 1:200 mTOR Cell Signaling, 2983 WB, 1:1000 p-Akt Ser-473 Cell Signaling, 4060 WB, 1:1000 p-mTOR Ser-2448 Cell Signaling, 5536 WB, 1:1000 p-p44/42 MAPK Cell Signaling, 4370 WB, 1:1000 p-S6 Ser-240/244 Cell Signaling, 5364 WB, 1:1000; IFC, 1:1000 p44/42 MAPK Cell Signaling, 9102 WB, 1:1000 p62 Cell Signaling, 5114 WB, 1:1000 S6 Cell Signaling, 2317 WB, 1:1000 -Actin Sigma, A2066 WB, 1:3000 20616 JOURNAL OF BIOLOGICAL CHEMISTRY VOLUME 289 • NUMBER 30 • JULY 25, 2014 at M em orial U niversity of N ew foundland on A ugust 1, 2014 http://w w w .jbc.org/ D ow nloaded from Transfections—The enhanced YFP plasmid was obtained from Clontech. .. Plasmids containing p70S6K (32) in competent DH5a E. coli were as follows: constitutively active p70S6K (Addgene plasmid 8993 pRK7-HA-S6K1-E389- CT), dominant negative p70S6K (Addgene plasmid 8986 pRK7-HAS6K1-F5A), wild-type p70S6K (Addgene plasmid 8984 pRK7HA-S6K1-WT), and the empty plasmid (Addgene 10883 pRK7) was used as a negative control.

    Plasmid Preparation:

    Article Title: Rapamycin and Interleukin-1β Impair Brain-derived Neurotrophic Factor-dependent Neuron Survival by Modulating Autophagy
    Article Snippet: .. Method and dilution Akt Cell Signaling, 9272 WB, 1:1000 Atg5 Sigma, A0731 WB, 1:500 Atg7 Abcam, ab53255 WB, 1:500 Cleaved caspase-3 Millipore, 9661 WB, 1:1000 HA Cell Signaling, 2367 IFC, 1:200 LC3 Cell Signaling, 3868 WB, 1:1000; IFC, 1:200 MAP2 Abcam, ab5392 IFC, 1:2000 Pan-Neuronal Marker Millipore, MAB2300 IFC, 1:200 mTOR Cell Signaling, 2983 WB, 1:1000 p-Akt Ser-473 Cell Signaling, 4060 WB, 1:1000 p-mTOR Ser-2448 Cell Signaling, 5536 WB, 1:1000 p-p44/42 MAPK Cell Signaling, 4370 WB, 1:1000 p-S6 Ser-240/244 Cell Signaling, 5364 WB, 1:1000; IFC, 1:1000 p44/42 MAPK Cell Signaling, 9102 WB, 1:1000 p62 Cell Signaling, 5114 WB, 1:1000 S6 Cell Signaling, 2317 WB, 1:1000 -Actin Sigma, A2066 WB, 1:3000 20616 JOURNAL OF BIOLOGICAL CHEMISTRY VOLUME 289 • NUMBER 30 • JULY 25, 2014 at M em orial U niversity of N ew foundland on A ugust 1, 2014 http://w w w .jbc.org/ D ow nloaded from Transfections—The enhanced YFP plasmid was obtained from Clontech. .. Plasmids containing p70S6K (32) in competent DH5a E. coli were as follows: constitutively active p70S6K (Addgene plasmid 8993 pRK7-HA-S6K1-E389- CT), dominant negative p70S6K (Addgene plasmid 8986 pRK7-HAS6K1-F5A), wild-type p70S6K (Addgene plasmid 8984 pRK7HA-S6K1-WT), and the empty plasmid (Addgene 10883 pRK7) was used as a negative control.



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    p mtor ser 2448 cell signaling  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc p mtor ser 2448 cell signaling
    P Mtor Ser 2448 Cell Signaling, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+mtor+ser+2448+cell+signaling/Glu-Glu+Tag+Antibody/pmc12478157__pnas%2E2414941122%2Esapp-51-49-52
    Average 96 stars, based on 1 article reviews
    p mtor ser 2448 cell signaling - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier

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